|
|
|
| Registros recuperados: 12 | |
|
|
| Brandão,Paulo Eduardo; Scheffer,Karin; Villarreal,Laura Yaneth; Achkar,Samira; Oliveira,Rafael de Novaes; Fahl,Willian de Oliveira; Castilho,Juliana Galera; Kotait,Ivanete; Richtzenhain,Leonardo José. |
| This article reports on the identification of a group 2 coronavirus (BatCoV DR/2007) in a Desmodus rotundus vampire bat in Brazil. Phylogenetic analysis of ORF1b revealed that BatCoV DR/2007 originates from a unique lineage in the archetypical group 2 coronaviruses, as described for bat species elsewhere with putative importance in Public Health. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coronavirus; Vampire bat; RNA-dependent; RNA-polymerase. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600003 |
| |
|
|
| Piatti,Rosa Maria; Ikuno,Alice Akimi; Cunha,Elenice Sequetin; D'Ambros,Régia; Gregori,Fábio; Soares,Rodrigo Martins; Cortez,Adriana; Richtzenhain,Leonardo José. |
| The genomic DNA of thirty strains of Aujeszky's disease virus (ADV) isolated in the South and Southeast regions of Brazil from 1982 to 1996 were characterized by restriction endonuclease analysis with BamHI. Twenty seven strains were isolated from pigs, 1 from cattle, 1 from cat and 1 from dog. Using a systematization previously described, the 30 ADV strains could be classified as genomic types I (n = 2) and II (n = 28). Genomic type III was not observed. In this first study of genomic type characterization of brazilian ADV strains, we could demonstrate the occurence in Brazil of the genomic types I and II, with a large predominance of genomic type II. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aujeszky's disease virus; Swine; Genomic RFLP; Brazil. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200015 |
| |
|
|
| Miraglia,Fabiana; Morais,Zenaide Maria; Cortez,Adriana; Melville,Priscilla Anne; Marvullo,Maria Fernanda Vianna; Richtzenhain,Leonardo José; Visintin,José Antonio; Vasconcellos,Silvio Arruda. |
| Inactivation of leptospires in pools of semen from three Holstein Friesian bulls, collected in an artificial vagina, was investigated. Spermatic concentration was adjusted in egg yolk citrate extender, submitted to the following treatments: A (control; without antibiotics); B (penicillin, 1,000 UI/mL - streptomycin, 1,000 µg/mL); C (amoxicillin, 1,000 µg/mL); D (ceptiofur sodium, 1,000 µg/mL); E (amoxicillin 1,000 µg/mL - ceptiofur sodium 1,000 µg/mL). Leptospires (2.0 x 10(6) leptospires/mL) were added into the diluted semen. Recovery of leptospires was obtained in modified EMJH semi-solid medium with and without antibiotics. The antibiotics in the concentrations used did not affect means of percentage of progressive motility and individual progressive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospires; Semen; Antibiotics; Extender. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200011 |
| |
|
| |
|
| |
|
|
| Silva,Teane Milagres Augusto da; Oliveira,Raquel Gonzaga de; Mol,Juliana Pinto da Silva; Xavier,Mariana Noyma; Paixão,Tatiane Alves da; Cortez,Adriana; Heinemann,Marcos Bryan; Richtzenhain,Leonardo José; Lage,Andrey Pereira; Santos,Renato de Lima. |
| Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Abortion; Brucella abortus; Salmonella sp.; Neospora caninum; Listeria monocytogenes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028 |
| |
|
|
| Cañón Franco,William Alberto; Bergamaschi,Denise Pimentel; Richtzenhain,Leonardo José; Nogueira,Yeda; Camargo,Luís Marcelo Aranha; Souza,Silvio Luís Pereira de; Gennari,Solange Maria. |
| Toxoplasmosis is a zoonosis that has been the subject of study in Brazil and worldwide. The dogs are sentinels for the infection and can carry Toxoplasma gondii in the environment. Seroepidemiological surveys of these animals are an important tool in the surveillance and control of the disease and inform decision-making in health programs. In this study the performance of the Modified Agglutination Test (MAT) in the serodiagnosis of canine toxoplasmosis is evaluated and compared to the indirect immunofluorescent-antibody test (IFAT). A sample of 157 dog sera from the county of Monte Negro, Rondônia, with 76.40% positive reactions for Toxoplasma gondii (IFAT =16) was analyzed using the MAT (=25), presenting sensitivity of 85.00% (Confidence Interval 95.00%:... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Toxoplasma gondii; Dog; Agglutination tests; Sensitivity and specificity. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000600009 |
| |
|
|
| Favaro,Patricia Filippsen; Fernandes,Wilson Roberto; Reischak,Dilmara; Brandão,Paulo Eduardo; Silva,Sheila Oliveira de Souza; Richtzenhain,Leonardo José. |
| Abstract Equine influenza is one of the major respiratory infectious diseases in horses. An equine influenza virus outbreak was identified in vaccinated and unvaccinated horses in a veterinary school hospital in São Paulo, SP, Brazil, in September 2015. The twelve equine influenza viruses isolated belonged to Florida Clade 1. The hemagglutinin and neuraminidase amino acid sequences were compared with the recent isolates from North and South America and the World Organisation for Animal Health recommended Florida Clade 1 vaccine strain. The hemagglutinin amino acid sequences had nine substitutions, compared with the vaccine strain. Two of them were in antigenic site A (A138S and G142R), one in antigenic site E (R62K) and another not in antigenic site... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine influenza; H3N8; Hemagglutinin; Neuraminidase; Evolutionary analysis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200336 |
| |
|
|
| Scarcelli,Eliana; Piatti,Rosa Maria; Harakava,Ricardo; Miyashiro,Simone; Fernandes,Flora Maria de Campos; Campos,Fabíola Ribeiro; Francisco,Waldemar; Genovez,Margareth Élide; Richtzenhain,Leonardo José. |
| The objective of the present trial was to characterize genetically strains of Campylobacter jejuni subsp. jejuni isolated from humans and several animal sources (bovines, swine, dogs, primates, wild boars and poultry). A total of 828 different animal samples (feces, carcass, aborted fetus and hysterectomized uterus) were analysed by means of routine bacteriological methods, and 36 C. jejuni strains were isolated. Thirty strains of human fecal origin were obtained in clinical analysis laboratories in the city of São Paulo. The 66 C. jejuni strains isolated were submitted to genetic characterization. Primers based on fla A gene were used in a polymerase chain reaction (PCR) and amplified a fragment of the 702 bp. PCR products were evaluated by means of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni subtyping sequencing; Flagellin. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400014 |
| |
|
|
| Nishi,Sandra Mayumi; Richtzenhain,Leonardo José; Gennari,Solange Maria. |
| Dois experimentos foram realizados para se conhecer a dinâmica dos anticorpos séricos (IgG) em bezerros após infecção com Haemonchus placei. No Experimento 1 estudou-se os níveis de IgG ao longo da primeira e segunda infecção. No Experimento 2 a influência de diferentes níveis protéicos da dieta e a resposta à infecção, medida através dos níveis de IgG séricos, foi observada em bezerros imunizados e não imunizados, através de infecções sucessivas e experimentalmente desafiados com L3 de H. placei. Observou-se que os bezerros infectados desenvolveram resistência a reinfecções, com acentuado decréscimo nos valores de ovos por grama de fezes (OPG) na segunda infecção. Entretanto, nesse mesmo período, os níveis de IgG séricos mantiveram-se elevados,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Haemonchus; Bovinos; ELISA. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000200010 |
| |
|
|
| Alves,Luciane Martins; Soares,Rodrigo Martins; Cortez,Adriana; Richtzenhain,Leonardo José; Ito,Fumio Nonma. |
| Hamsters orally inoculated with ERA and PV strains of rabies virus were sacrificed at 24, 48, 72 hours, 21 and 30 days after inoculation. Brain fragments were examined by Fluorescent Antibody test (FAT) and heminested PCR (hn-PCR). Fragments from stomach, blood, heart, and lung were examined only by hn-PCR. Sera of other hamsters, similarly inoculated, obtained at 30th day after inoculation were submitted to mouse neutralization test. The hamsters were challenged intracerebrally with CVS strain with 10(2.7)mouse LD50/0.03mL, 45 days after inoculation. Brains examined by FAT were negative. The hn-PCR detected the presence of rabies virus RNA in the lung of one animal inoculated with ERA, and in the brain, stomach, blood, and lung of PV-infected animals. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabies; Oral administration; ERA and PV strains; Hn-PCR; Hamster. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000100010 |
| |
|
|
| Heinemann,Marcos Bryan; Cortez,Adriana; Souza,Maria do Carmo Custódio de; Gotti,Tatiana; Ferreira,Fernando; Homem,Valéria Stachini Ferreira; Ferreira Neto,José Soares; Soares,Rodrigo Martins; Sakamoto,Sidnei Mioshi; Cunha,Elenice Maria Sequetin; Richtzenhain,Leonardo José. |
| Os vírus da anemia infecciosa eqüina (VAIE), da arterite viral dos eqüinos (VAVE) e do aborto viral eqüino (Herpesvírus eqüino tipo 1, HVE-1) são agentes causadores de enfermidades nos eqüídeos que podem causar graves prejuízos econômicos. O objetivo do presente trabalho foi estimar a soroprevalência de anticorpos contra os vírus VAIE, VAVE e HVE-1, utilizando como unidades de análise os eqüídeos e as propriedades rurais do tipo familiar do município de Uruará, PA. Os anticorpos contra o VAIE foram pesquisados pela prova de imunodifusão em gel de ágar e os anticorpos contra o VAVE e o HVE-1 pela prova de soroneutralização. O tamanho da amostra foi estimado a partir de um total de 2069 propriedades, caracterizadas por agricultura familiar e ausência de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prevalência; Uruará (PA); Anemia infecciosa eqüina; Arterite animal. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100009 |
| |
| Registros recuperados: 12 | |
|
|
|
